Enhanced production of prodigiosin by Serratia marcescens FZSF02 in the form of pigment pellets

Background: Prodigiosin has been demonstrated to be an important candidate in investigating anticancer drugs and in many other applications in recent years. However, industrial production of prodigiosin has not been achieved. In this study, we found a prodigiosin-producing strain, Serratia marcescens FZSF02, and its fermentation strategies were studied to achieve the maximum yield of prodigiosin. Results: When the culture medium consisted of 16.97 g/L of peanut powder, 16.02 g/L of beef extract, and 11.29 mL/L of olive oil, prodigiosin reached a yield of 13.622 ± 236 mg/L after culturing at 26 °C for 72 h. Furthermore, when 10 mL/L olive oil was added to the fermentation broth at the 24th hour of fermentation, the maximum prodigiosin production of 15,420.9 mg/L was obtained, which was 9.3-fold higher than the initial level before medium optimization. More than 60% of the prodigiosin produced with this optimized fermentation strategy was in the form of pigment pellets. To the best of our knowledge, this is the first report on this phenomenon of pigment pellet formation, which made it much easier to extract prodigiosin at low cost. Prodigiosin was then purified and identified by absorption spectroscopy, HPLC, and LCMS. Purified prodigiosin obtained in this study showed anticancer activity in separate experiments on several human cell cultures: A549, K562, HL60, HepG2, and HCT116. Conclusions: This is a promising strain for producing prodigiosin. The prodigiosin has potential in anticancer medicine studies.

Valor nutritivo de gramíneas do gênero Cynodon consorciadas com amendoim forrageiro / Nutritive value of grasses of genus Cynodon mixed with forage peanut

O objetivo do presente estudo foi avaliar o valor nutritivo do consórcio forrageiro entre gramíneas do gênero Cynodon (cv. tifton 85 ou coastcross) com Arachis pintoi (amendoim forrageiro). O experimento foi conduzido na Universidade Tecnológica Federal do Paraná, Câmpus Dois Vizinhos, em área de aproximadamente 3000m2. O delineamento experimental foi de blocos ao acaso, com quatro tratamentos e três repetições. Os tratamentos foram: tifton 85 e coastcross em cultivo estreme, tifton 85 + AF e coastcross + AF. Foram avaliados os teores de PB, FDN, FDA, DIVMS, MM e MS das lâminas foliares, colmo+bainha, amendoim forrageiro e da MF disponível da simulação de pastejo. Foi observado que a coastcross tem menor teor de fibra, independentemente do tratamento. O avanço das estações do ano proporcionou menores valores de PB e DIVMS em todos os tratamentos. A simulação de pastejo demonstrou que a tifton 85 tem maiores teores de MS, assim como valores inferiores são encontrados para os consórcios. Quando consorciado com as gramíneas, o amendoim forrageiro reduziu os compostos fibrosos na pastagem, acrescentando maiores teores de PB em pastagem de gramíneas no final do ciclo produtivo.(AU)

The goal of this study was to evaluate the fodder performance and production of Cynodon (Cv. Tifton 85 or Coastcross) mixed with Arachis pintoi (Forage peanut). The experiment was conducted at the Universidade Tecnológica Federal do Paraná, Câmpus Dois Vizinhos, at an area of approximately 3000m2. A completely randomized block design was applied, with four treatments and three replicates. The treatments were: Tifton 85 and Coastcross in pure crop, Tifton 85 + AF and Coastcross + AF with rotated and successive implantation of one line to the grass and three consecutive lines to the legume. The content of CP, NDF, ADF, IVDMD ashe and DM of the leaf blades, stem of grasses forage peanut and total forage mass available of grazing simulation was evaluated. Coastcross has lower fibrous contents, regardless of treatment. The changing of the seasons during the year provided lower values of CP and IVDMD for all treatments. The evaluation of the grazing simulation showed that Tifton 85 has higher DM values just as the lower ones are found for consortia. When mixed with grasses, forage peanuts reduce the fibrous compounds on pasture, increasing the CP levels in grass pasture at the end of productive cycle.(AU)

Promotion of iron nutrition and growth on peanut by Paenibacillus illinoisensis and Bacillus sp. strains in calcareous soil

ABSTRACT This study aimed to explore the effects of two siderophore-producing bacterial strains on iron absorption and plant growth of peanut in calcareous soil. Two siderophore-producing bacterial strains, namely, YZ29 and DZ13, isolated from the rhizosphere soil of peanut, were identified as Paenibacillus illinoisensis and Bacillus sp., respectively. In potted experiments, YZ29 and DZ13 enhanced root activity, chlorophyll and active iron content in leaves, total nitrogen, phosphorus and potassium accumulation of plants and increased the quality of peanut kernels and plant biomass over control. In the field trial, the inoculated treatments performed better than the controls, and the pod yields of the three treatments inoculated with YZ29, DZ13, and YZ29 + DZ13 (1:1) increased by 37.05%, 13.80% and 13.57%, respectively, compared with the control. Based on terminal restriction fragment length polymorphism analysis, YZ29 and DZ13 improved the bacterial community richness and species diversity of soil surrounding the peanut roots. Therefore, YZ29 and DZ13 can be used as candidate bacterial strains to relieve chlorosis of peanut and promote peanut growth. The present study is the first to explore the effect of siderophores produced by P. illinoisensis on iron absorption.

Análise do extrato aquoso de Arachis hipoagea L. no combate à dislipidemia e ao ganho ponderal de ratos Wistar submetidos à dieta hiperlipídica / Analysis of the aqueous extract of Arachis hipoagea L. to reduce dyslipidemia and weight gain in Wistar rats with high fat diet

O objetivo desse estudo foi avaliar os efeitos do Extrato Aquoso de Amendoim (EAA) no peso, bioquímica sérica e na histologia hepática de ratos Wistar submetidos a dietas normo e hiperlipídicas. A pesquisa foi realizada utilizando 40 ratos Wistar machos, divididos em quatro grupos (n=10): GA (dieta hiperlipídica), GB (dieta hiperlipídica +EAA), GC (dieta normolipídica) e GD (dieta normolipídica +EAA). Após 8 semanas, os animais foram eutanasiados e foram coletadas amostras sanguíneas para a avaliação de dados bioquímicos (Colesterol total e suas frações, triglicerídeos, uréia, creatinina, AST, ALT e glicemia) e fragmentos do fígado para análise histológica. Os animais do grupo GB tiveram um ganho de peso inferior quando comparados ao GA (XGB= versus XGA= p<0,05), já os grupos GC e GD não obtiveram diferenças estatísticas. Os animais que receberam o EAA tiveram uma redução nos níveis de colesterol (XGB= versus XGA= p<0,05 e XGD= versus XGA= p<0,01), dos triglicerídeos (XGB= versus XGA e XGD= versus XGA= p<0,001) e mais discretamente dos níveis de ALT. A glicemia, uréia e creatina permaneceram dentro dos valores de referência. As amostras hepáticas analisadas, dos ratos dos diferentes grupos, não apresentaram alterações histopatológicas. Conclui-se que O EAA apresentou efeitos preventivos sobre o ganho ponderal e dislipidemia.(AU)

The aim of this study was to evaluate the effect of Aqueous Extract from Peanut (EAA) in weight, serum biochemistry and liver histology of Wistar rats with normal hearing and a high fat diet. The survey was conducted using 40 male Wistar rats divided into four groups (n=0): GA (high fat diet), GB (fat diet+EAA), GC (normolipídica diet), and GD (normolipídica diet+EAA). After 8 weeks, the rats were euthanized and blood samples were collected to evaluate biochemical data (total cholesterol and its fractions, triglycerides, urea, creatinine, AST, ALT and glucose) and liver fragments for histological analysis. The animals of the GB group had a lower weight gain when compared with GA (XGB versus XGA= p<0.05), but CG and GD did not obtain statistical differences. The rats that received EAA had a reduction in cholesterol levels (XGB= versus XGA= p<0.05 vs. XGA and XGD= p<0.01), triglycerides (=XGB versus XGA and =XGD versus XGA= p<0,001) and more discreetly ALT levels. Blood glucose, urea and creatine remained within the reference values. Liver samples analyzed, the rats of different groups showed no histopathological changes. In conclusion, the EAA had preventive effects on weight gain and dyslipidemia.(AU)

Synergic actions of polyphenols and cyanogens of peanut seed coat (Arachis hypogaea) on cytological, biochemical and functional changes in thyroid.

In animals, long-term feeding with peanut (Arachis hypogaea) seed coats causes hypertrophy and hyperplasia of the thyroid gland. However, to date there have been no detailed studies. Here, we explored the thyroidal effects of dietary peanut seed coats (PSC) in rats. The PSC has high levels of pro-goitrogenic substances including phenolic and other cyanogenic constituents. The PSC was mixed with a standard diet and fed to rats for 30 and 60 days, respectively. Animals fed with the PSC-supplemented diet showed a significant increase in urinary excretion of thiocyanate and iodine, thyroid enlargement, and hypertrophy and/or hyperplasia of thyroid follicles. In addition, there was inhibition of thyroid peroxidase (TPO) activity, 5’-deiodinase-I (DIO1) activity, and (Na+-K+)-ATPase activity in the experimental groups of rats as compared to controls. Furthermore, the PSC fed animals exhibited decreased serum circulating total T4 and T3 levels, severe in the group treated for longer duration. These data indicate that PSC could be a novel disruptor of thyroid function, due to synergistic actions of phenolic as well as cyanogenic constituents.

A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L)

The high oleic (C18:1) phenotype in peanuts has been previously demonstrated to result from a homozygous recessive genotype (ol1ol1ol2ol2) in two homeologous fatty acid desaturase genes (FAD2A and FAD2B) with two key SNPs. These mutant SNPs, specifically G448A in FAD2A and 442insA in FAD2B, significantly limit the normal function of the desaturase enzyme activity which converts oleic acid into linoleic acid by the addition of a second double bond in the hydrocarbon chain. Previously, a genotyping assay was developed to detect wild type and mutant alleles in FAD2B. A real-time PCR assay has now been developed to detect wild type and mutant alleles (G448A) in FAD2A using either seed or leaf tissue. This assay was demonstrated to be applicable for the detection of homozygous and heterozygous samples. The FAD2A genotyping assay was validated by employing gas chromatography (GC) to determine total fatty acid composition and by genotyping peanut lines that have been well characterized. Overall, development of rapid assays such as real-time PCR which can identify key genotypes associated with important agronomic traits such as oleic acid, will improve breeding efficiency by targeting desirable genotypes at early stages of development.

Calidad nutricional en variedades de maní tegua y granoleico: estabilidad y aceptabilidad de sus aceites: efecto de su ingesta sobre niveles de lípidos plasmáticos en ratones / Nutritional quality tegua peanut varieties and granola: stability and acceptability of their oils: effect of intake on plasma lipid levels in mice

El maní es una de las principales oleaginosas del mundo y Argentina es uno de sus principales productores y exportadores. En la provincia de Córdoba se concentra el 94,63% del cultivo. Las variedades del tipo Runner: cultivar Tegua y Granoleico, presentan perfiles muy diferentes de ácidos grasos. El alto contenido en ácido graso nonoinsaturado en los productos derivados de maní lo hace muy estable ante los procesos de oxidación y altamente beneficioso para la prevención de enfermedades cardiovasculares. El objetivo de este estudio fue valorar la calidad nutricional de los maníes de las variedades Tegua (T) y Granoleico (GO) mediante el análisis de la estabilidad y aceptabilidad de sus aceites y en especial la evaluación de sus efectos sobre los lípidos plasmáticos en ratones Albino swiss. Materiales y métodos: Se utilizaron granos de maní de las variedades citadas, se determinaron macronutrientes, humedad, minerales y tocoferoles. Se obtuvo aceite de maní por método de prensado en frío. Se evaluó la composición de ácidos grasos y se la comparó con las de otros aceites vegetales (girasol, maíz, soja, canola y oliva). Estabilidad. Se analizaron índice de peróxido(IP), índice de p‐anisidina (IA) y dienos conjugados (DC) en aceites puros (T‐GO 100‐0 y T‐GO 0‐ 100) y en mezclas de las variedades: al 25% (T‐GO 75‐25), al 50% (T‐GO 50‐50) y al 75% (T‐GO 25‐ 75) almacenados en estufa a 60 ºC. Lípidos plasmáticos. Se realizó un modelo experimental conratones Albino swiss (n=81), se utilizaron dietas semisintéticas con la adición de aceites refinados de girasol, oliva, maní GO y dieta comercial. Se controló el peso semanalmente. A los 77 y 126 días de tratamiento se los sacrificó y midieron los lípidos plasmáticos: triglicéridos, colesterol total, cHDL y cLDL. Análisis sensorial. Se comparó la aceptabilidad de los aceites de maní, girasol y oliva extravirgen. Estadística. Varianza, test de LSD Fisher (α=0,05) y regresión.

Biosorption of lead(II) and chromium(VI) on groundnut hull: equilibrium, kinetics and thermodynamics study / Biosorción de plomo (II) y cromo (VI) en cáscaras de maní: estudio de equilibrio, cinética y termodinámica

The biosorption of lead(II) and chromium(VI) on groundnut hull was investigated. Batch biosorption experiments were conducted to find the equilibrium time and biosorption capacity. Effect of parameters like pH, temperature and initial metal concentration was studied. The maximum biosorption capacity of lead(II) and chromium(VI) was found to be 31.54 +/- 0.63 and 30.21 +/- 0.74 mg g-1, respectively. The optimum pH for lead(II) and chromium(VI) removal was 5 ± 0.1 and 2 +/- 0.1, respectively. The temperature change, in the range of 20 - 45ºC affected the biosorption capacity. The maximum removal of lead(II) was achieved at 20 +/- 2ºC, where as maximum uptake of chromium(VI) was observed at 40 +/- 2ºC. The biosorption data was fitted to the Langmuir and the Freundlich isotherm models. The Langmuir model showed better representation of data, with correlation coefficient greater than 0.98. The kinetics of biosorption followed the pseudo second order kinetics model. The thermodynamics parameters were evaluated from the experimental data.

A rapid and cheap protocol for preparation of PCR templates in peanut

This paper describes a simple, low cost and reliable DNA template preparation protocol for polymerase chain reaction (PCR) using immature leaves from peanut seeds or leaves from field-grown plants. The technique may find wide utility in studies involving PCR-based molecular markers, rapid screening for transformants and gene cloning.

Determination of aflatoxins in nuts, peanuts, almonds and hazelnuts: identification of producing fungi

The objective of the present work was to analyse nuts, peanuts, almonds and hazelnuts samples to detect the presence of aflatoxins B1 B2 G1 and G2. We also studied the fungic contamination of the above mentioned foodstuffs because fungi the produce the aflatoxins are frequent environmental contaminants the may grow in a wide variety of substrates and under various conditions of humidity, temperature and pH. Therefore, these foodstuffs may suffer a fungic invasion during their production, processing, transportation or storing. Isolated fungi were also studied to find out their capacity for producing toxins in an adequate medium. Out of a total of 100 samples, in two of them, corresponding to peanuts, the presence of Aflatoxin B1 was detected in a concentration of 21 mg/kg and 39 mg/kg respectively. 31 strains of Aspergillus were isolated. 26 isolations correspond to Aspergillus flavus, while 5, to Aspergillus parasiticus. Out of the said 31 strains, 6 were found to produce aflatoxin B in an appropriate culture medium. This allows us to handle more information about the presence of mycotoxins in foodstuffs, to prevent fungic contamination in early stages and alert the public to fungi toxicity.

Características nutricionales de barras de cereales y maní / Nutritional characteristics of cereal and peanut bars

La importancia de los "snack" de buen valor nutricional, radica en que los niños y adolesscentes presentan gran preferencia por este tipo de productos los que podrán cumplir una función destacada en su desarrollo físico y mental. La tendencia es aumentar su valor nutritivo entregando proteínas, carbohidratos, fibra vitaminas y minerales en forma balanceada. El objetivo de este trabajo fue evaluar la calidad química, sensorial y nutricional de barras elaboradas con mezclas de cereales y maní. Se prepararon 3 barras utilizando avena, germen de trigo, maní, amaranto tostado, amaranto dilatado y extruido de trigo en diferentes proporciones. Se analizó la composición proximal de las barras de acuerdo a los métodos de la A.O.A.C. y su aceptabilidad según Escala Hedónica. Para los análisis biológicos se utilizaron ratas alimentadas con dietas al 10 por ciento de proteínas, se determinó Razón de Eficiencia Proteica (PER), Razón Proteica Neta (NPR) y digestibilidad aparente (DAP). Con los datos obtenidos se calculó PER corregido, PER relativo, NPR relativo y DAP relativa. Los valores de PER y NPR demostraron que no hubo diferencias entre las barras CM1 y CM2 (PER 2,59-2,57; NPR 3,99-3,95 respectivamente); pero si con la CM3 que presentó una calidad inferior. En cuanto al DAP no hubo diferencias significativas entre las barras. Las barras CM1 y CM2 presentaron la mejor calidad biológica de la proteína, siendo la barra CM3 de inferior calidad. Desde el punto de vista químico y sensorial la barra CM1 presentó el má alto contenido de proteína (14,23 por ciento); y la mayor aceptabilidad (6,8) y la barra CM2 presentó un alto aporte de fibra cruda (2,27 por ciento).

Almacenamiento y uso de antioxidantes en barras de cereales y maní / Storage and use of antioxidants in cereal and peanut bars

La utilización de materias grasas en las barras de cereales les da un adecuado valor energético pero también las expone al desarrollo de rancidez oxidativa, la cual puede afectar su aceptabilidad y calidad nutricional. De acuerdo a esto, el objetivo del presente trabajo fue: determinar la estabilidad en almacenamiento y el efecto de antioxidantes en tres tipos de barras de cereales y maní. Para ello se elaboraron barras de cereales con 18 por ciento de maní, con y sin antioxidantes (BHA+BHT; 100 ppm). Las barras se envasaron en bolsas de polipropileno -aluminio- polietileno y se almacenaron por 90 días a temperatura ambiente (18-20 grados Celsius). Cada 30 días se realizaron análisis de: Actividad de agua (Aw); Humedad; Indice de peróxido y Evaluación sensorial para determinar su calidad (aroma, sabor y apariencia) y aceptabilidad. El contenido de humedad fue similar en todas las barras (7,6-9,5 por ciento) en tanto que Aw fue superior en las barras con amaranto dilatado y antioxidante. El índice de peróxidos a los 60 días de almacenamiento fue menor que las barras con antioxidantes, presentando diferencias significativas solo la barra con amaranto dilatado (16,4 meq/Kg para la barra con antioxidante y 25,7 meq/Kg para la barrra testigo). En cuanto a la calidad sensorial los valores de los tres parámetros se mantuvieron dentro de los rangos normales, sin diferencias entre las barrras testigo y las barras con antioxidante y sin variaciones durante el período de almacenamiento. La vida útil de las barras CM1 y CM2 es al menos de 60 días cuando son conservadas a 18-20 grados Celsius.

Efeito do forno de microondas na destruiçäo de aflatoxinas em amendoim / Microwave oven effect on the destruction of aflatoxins on peanuts

Foi verificado o efeito do aquecimento de amostras contaminadas artificialmente com aflatoxinas B1 e G1, pelo forno de microondas, em potência máxima de 0,8kW, por 3 a 6 minutos. A quantificaçäo de aflatoxinas foi desenvolvida por cromatografia em camada delgada por comparaçäo visual com padröes. Os resultados encontrados mostraram uma reduçäo de 41,52 a 69,56(por cento) de aflatoxinas B1 e G1 em amostras torradas por 6 minutos.

Purification and partial characterization of acyl carrier proteins from developing oil seeds of pisa (Actinodaphne hookeri) and ground nut (Arachis hypogaea).

Acyl carrier proteins (ACP) were purified to homogeneity in the active form from developing seeds of pisa (Actinodaphne hookeri) which synthesizes exclusively trilaurin and from ground nut (Arachis hypogaea) which synthesizes triacylglycerols containing long chain fatty acids. Two major isoforms of ACPs were purified from developing pisa seeds using DEAE-cellulose, Superose-6 FPLC and C4 reversed phase HPLC chromatographic methods. In contrast, only a single form of ACP was present in ground nut seeds which was purified by anion-exchange and activated thiol-Sepharose 4B affinity chromatography. The two isoforms of ACPs from pisa showed nearly the same specific activity of 6,706 and 7,175 pmol per min per mg protein while ground nut ACP showed a specific activity of 3,893 pmol per min per mg protein when assayed using E. coli acyl-ACP synthetase and [1-14C]palmitic acid. When compared with E. coli ACP, the purified ACPs from both the seeds showed considerable difference in their mobility in native PAGE, but showed similar mobility in SDS-PAGE under reducing conditions. In the absence of reducing agents formation of dimers was quite prominent. The ACPs from both the seed sources were acid- and heat-stable. The major isoform of pisa seed ACP and the ground nut ACP contain 91 amino acids with M(r) 11,616 and 1,228 respectively. However, there is significant variation in their amino acid composition. A comparison of the amino acid sequence in the N-terminal region of pisa and ground nut seed ACPs showed considerable homology between themselves and with other plant ACPs but not with E. coli ACP.

Chemical composition of drug legume seeds grown in three different countries

Dry mature seeds of fourteen cultivars or lines of legumes produced in three different countries were compared with respect to their mono and oligosaccharide contents in an attempt to determine the possibilities for selection in a breeding programme on the basis of some chemical characteristics. Materials were obtained from legume production areas in Egypt, U.S.A. and India. Egyptian legumes included Faba bean [Giza 2], Mung bean [T 44]. Soybean [Crowford], Pea-nut [Giza 5] and Lentil [Giza 9]. The American legumes were Faba bean, Navy bean, Soybean, Kidney bean, Black eye peas and lentils. The Indian legume included pengel gram, Red gram and Green gram. The determination of total dietary fiber [TDF] of the whole dry seed range from 5.5% to l3.2% for Egyptian legumes and from 7.73% to 17.95% for American legumes, in case of Indian legumes range from 6.13% to 13.67%. The rafinose content of the whole dry seed range from 0.11% to 0.44% for Egyptian legume, from 0.11% to 1.35% for American legumes and from 0.36% to 0.53% for Indian legumes on dry matter basis, while sucrose range from 1.3% to 1.8% for American legumes, from 1.3% to 1.5 for Indian legumes and from 0.96% to 2.09% for Egyptian legumes

Screening of various legumes for their cyanide content

Various types of legumes were collected from three different places. Five legumes from Egypt [Faba bean, Mung bean, Lentils, Soybean and Peanut]. Six legumes from U. S. A. [Kidney bean, Navy bean, Soybean, Faba bean, Black eye bean and Lentils] and three legume from India [Bengo gram, Red grain and Green gram]. In these fourteen types of legume, free and bound hydrocyanide were measured by using spectroquant 14800 Kits and total hydrocyanide was calculated. The results showed that the American Soybean was contained highest amount of free HCN than all other legumes while Red gram of Indian legumes gave the lowest amount of free hydrocyanide. However Green gram of Indian legumes was contained highest amount of bound hydrocyanide, the Black eye bean was contained lowest amount of bound hydrocyanide, in case of total cyanide the soybean, and pea-nut were contained highest amount of CN[-] and also Black eye bean was contained lowest amount of CN[-]

Barras de cereales maní y amaranto dilatado; composición química y estabilidad en almacenamiento acelerado / Cereal bars with peanut and puffed amaranthus: chemical composition and stability in accelerated storage

Usando la línea de flujo de Escobar et al, se prepararon 3 formulaciones CM3A-CM3B y CM3C con diferentes cantidades de avena, germen de trigo y amaranto dilatado; para su aglutinación se emplearon edulcorantes naturales y materias grasas. A las materias primas y a los "snacks" se les hizo análisis químico proximal. Los snacks se evaluaron sensorialmente para conocer su calidad y aceptabilidad. Para determinar la estabilidad de las barras durante el almacenamiento acelerado, estas se conservaron en estufa a 37ºC por 15 días, se analizaron cada 5 días para medir la evolución de actividad de agua (Aw), humedad, desarrollo de peróxidos y se controló sensorialmente la aparición de rancidez. La aceptabilidad de la CM3B fue de 91,67 por ciento con 0.0 por ciento de rechazo, CM3A y CM3C obtuvieron aceptabilidad (66,67 por ciento) y 8.33 por ciento de rechazo. En el almacenamiento acelerado Aw y la humedad disminuyeron hasta llegar a un valor de 0.48 y 5.9 respectivamente en la barra CM3B, el contenido de peróxido en las barras CM3B y CM3C aumentó gradualmente hasta llegar a los 15 días a 12 y 17 meq/kg respectivamente. El aroma a rancio no siguió un patrón común en las 3 barras ya que CM3A y CM3B tuvieron una disminución a los 15 días no así las barras CM3C. En síntesis se puede decir que la barra CM3B presentó la mejor calidad sensorial, la más alta aceptabilidad y la mejor estabilidad en el almacenamiento acelerado